Safe Genetic Modification of Cardiac Stem Cells Using a Site-Specific Integration Technique
نویسندگان
چکیده
منابع مشابه
Safe genetic modification of cardiac stem cells using a site-specific integration technique.
BACKGROUND Human cardiac progenitor cells (hCPCs) are a promising cell source for regenerative repair after myocardial infarction. Exploitation of their full therapeutic potential may require stable genetic modification of the cells ex vivo. Safe genetic engineering of stem cells, using facile methods for site-specific integration of transgenes into known genomic contexts, would significantly e...
متن کاملCell Transplantation and Tissue Regeneration Safe Genetic Modification of Cardiac Stem Cells Using a Site-Specific Integration Technique
Background—Human cardiac progenitor cells (hCPCs) are a promising cell source for regenerative repair after myocardial infarction. Exploitation of their full therapeutic potential may require stable genetic modification of the cells ex vivo. Safe genetic engineering of stem cells, using facile methods for site-specific integration of transgenes into known genomic contexts, would significantly e...
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To date, a large number of reports have described reprogramming many somatic cell types into induced pluripotent stem (iPS) cells, using different numbers of transcription factors and devising alternate methods of introducing the transcription factor genes or proteins into the somatic cells. Here, we describe a method using bacteriophage ΦC31 integrase to reprogram mouse embryonic fibroblasts a...
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Some of the illnesses that plague man kind are directly related to our genes. Severe genetic disorders with no current therapeutic options—immuno deficiencies, haemophilia, thalassaemia, muscular dystrophies and cystic fibrosis, for example—could potentially be treated with gene therapy, which uses genetic informa tion to correct a mutation or to provide new functions for human cells. the ai...
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Abbreviations: Ad: adenovirus, bFGF: basic fibroblast growth factor, BMP: bone morphogenetic protein, CNS: central nervous system, ES: embryonic stem, FACS: fluorescence activated cell sorting, HIV: human immunodeficiency virus, ICM: inner cell mass, LIF: leukemia inhibitory factor, MEF: mouse embryonic fibroblast, RNAi: RNA interference, SCNT: somatic cell nucleus transfer, UTR: untranslated r...
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ژورنال
عنوان ژورنال: Circulation
سال: 2012
ISSN: 0009-7322,1524-4539
DOI: 10.1161/circulationaha.111.084913